Value of SNP-Array technique in genetic analysis of abortion tissue

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Author: Wei-Yun ZHANG ¹ Yu-Min WANG ¹ Xiu-Qin LI ¹  Feng-Qi LI ²

Affiliation: 1. Zhoukou Maternal and Child Health Hospital (Municipal Children's Hospital), Zhoukou 466000, Henan Province, China 2. Zhoukou Institute of Pediatrics, Zhoukou 466000, Henan Province, China

Keywords: Single nucleotide polymorphism microarray Genetics Chromosome Abortion tissue

DOI: 10.12173/j.issn.1004-4337.202212004

Reference: Zhang WY, Wang YM, Li XQ, Li FQ. Value of SNP-Array technique in genetic analysis of abortion tissue[J]. Journal of Mathematical Medicine, 2023, 36(1): 55-60. DOI: 10.12173/j.issn.1004-4337.202212004.[Article in Chinese]  Copied

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Abstract

Objective To investigate the value of single nucleotide polymorphism microarrays (SNP-arrays) in the genetic analysis of abortion tissues.

Method A total of 50 patients diagnosed with missed miscarriage in Zhoukou Maternal and Child Health Hospital from July 2020 to February 2022 were selected as study subjects, and numbered in the order of patient inclusion. Each patient was detected separately using SNP-array technology (SNP-array group) and fluorescence in situ hybridization technique (control group) to compare the detection of chromosomal abnormalities.

Result There were 34 cases of chromosomal abnormalities, 22 cases of euploidy abnormalities and 12 cases of aneuploidy abnormalities in the SNP-array group, and 24 cases, 15 cases and 9 cases in the control group, respectively. The detection rate of chromosomal abnormalities in the SNP-array group (68.00%) was higher than that in the control group (48.00%), and the difference was statistically significant (X²=4.105, P=0.043). There were 9 cases of SNP-array microduplicate microdeletions, including single-point duplication on No.33, single-point deletion on No.7, two-point repeat on No.2, two-point deletion on No.41, and the remaining 5 microduplicate microdeletions with 2 sites or more.

Conclusion SNP-array technology has a high detection rate of abnormal chromosomes in the genetic analysis of abortion tissue, and has good clinical application value.

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