Objective  To explore the best technology for preparing ginsenoside Rg5 by citric acid hydrolysis from total ginsenoside of ginseng stem and leaf.

Methods  The orthogonal test and Box-Behnken response surface method were used to optimize the solid-liquid ratio, citric acid concentration, reaction time and reaction temperature. The quadratic mathematical model of ginsenoside Rg5 preparation process by citric acid hydrolysis was established and its reliability was verified.

Results  After the optimization of orthogonal test and Box-Behnken response surface, the optimal process was as follows: the solid-liquid ratio was 1∶21, the citric acid concentration was 30%, the reaction time was 3 h, the reaction temperature was 95  ℃. Under these conditions, the average yield of ginsenoside Rg5 was 9.64%.

Conclusion  The process conditions optimized by orthogonal test and Box-Behnken response surface method being applied to ginsenoside Rg5 preparation process had higher conversion efficiency, which provided data support for the development and utilization of rare ginsenoside components.

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