Objective  To explore the optimal water extraction technology of polysaccharides from American ginseng flower and establish a method for the content determination of polysaccharides in American ginseng flower.

Methods  Water extraction method was used to extract polysaccharides from American ginseng flower, single factor experiment was used to optimize the extraction temperature. In the orthogonal experiment, the extraction rate of polysaccharides from American ginseng flower was used as the index to optimize the process parameters. The content of polysaccharides in American ginseng flower was determined by phenol-sulfuric acid method, and the methodology research was conducted.

Results  The optimum extraction conditions were as follows: at 95 ℃, heating for 2.5 hours, extracted 3 times, the solid-liquid ratio was 1∶20 (multiple). The extraction rate of polysaccharide from American ginseng flower was 7.01%, the extraction rate of ginseng flower polysaccharide was 6.54%. The content of polysaccharides from American ginseng flower was higher than that from ginseng flower. The results of methodological trials showed that D-anhydrous glucose showed a good linear relationship with absorbance between 65 μg and 325 μg, and the average recovery was 99.07%.

Conclusion  The extraction efficiency of this process was high, the detection method was stable and feasible, and it could provide experimental basis for further analysis of polysaccharide components and physiological evaluation of American ginseng flower.

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